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小鼠還原型谷胱甘肽酶免試劑盒,(GSH)ELISA檢測試劑盒 ,英文名:GSH ELISA Kit ,英文縮寫:GSH ,常見試劑盒規(guī)格:96T/48T,產品別名:小鼠還原型谷胱甘肽ELISA檢測試劑盒,GSH 檢測試劑盒, GSH 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。小鼠還原型谷胱甘肽酶免試劑盒,(GSH)ELISA檢測試劑盒在操作中應注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應和酶促反應時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關鍵步驟。目的是除去未結合的免疫反應物,終止抗原抗體反應,除去標本中與反應無關的成分、游離的酶標物以及反應過程中吸附于固相載體上的非特異性物質。可用洗板機洗板或手工洗板,前者洗滌質量較好,各反應孔洗滌效果均一,批內、批間差異小,手工洗板應注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據反應液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應孔的吸光度值(A值)。酶標儀具有良好的重復性。
激肽釋放酶14(KLK14)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 14 (KLK14)48T/96TY12885Homo sapiens (Human)
精氨酸酶Ⅱ(Arg2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Arginase II (Arg2)48T/96TY12886Homo sapiens (Human)
肽聚糖識別蛋白1(PGLYRP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Peptidoglycan Recognition protein 1 (PGLYRP1)48T/96TY12887Homo sapiens (Human)
分泌粒蛋白Ⅱ(SCG2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Secretogranin II (SCG2)48T/96TY12888Homo sapiens (Human)
鈣調寧蛋白2(CNN2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Calponin 2 (CNN2)48T/96TY12889Homo sapiens (Human)
EGF樣域蛋白7(EGFL7)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for EGF Like Domain Protein, Multiple 7 (EGFL7)48T/96TY12890Mus musculus (Mouse)
T-細胞免疫調節(jié)因子1(TCIRG1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for T-Cell, Immune Regulator 1 (TCIRG1)48T/96TY12891Homo sapiens (Human)
醛糖還原酶相似蛋白1(ARL1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aldose Reductase Like Protein 1 (ARL1)48T/96TY12892Homo sapiens (Human)
胞外5'-核苷酸酶(NT5E)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 5'-Nucleotidase, Ecto (NT5E)48T/96TY12893Homo sapiens (Human)
胰島素樣生長因子1受體(IGF1R)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Insulin Like Growth Factor 1 Receptor (IGF1R)48T/96TY12894Homo sapiens (Human)
S100鈣結合蛋白A5(S100A5)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for S100 Calcium Binding Protein A5 (S100A5)48T/96TY12895Homo sapiens (Human)
纖維蛋白原γ(FGγ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibrinogen Gamma (FGg)48T/96TY12896Homo sapiens (Human)
促腎上腺皮質素釋放激素受體1(CRHR1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Corticotropin Releasing Hormone Receptor 1 (CRHR1)48T/96TY12897Rattus norvegicus (Rat)
5-羥色胺受體2C(HTR2C)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for 5-Hydroxytryptamine Receptor 2C (HTR2C)48T/96TY12898Rattus norvegicus (Rat)
腎上腺髓質素受體(AMR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Adrenomedullin Receptor (AMR)48T/96TY12899Homo sapiens (Human)
翻譯調節(jié)腫瘤蛋白1(TPT1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Tumor Protein, Translationally Controlled 1 (TPT1)48T/96TY12900Mus musculus (Mouse)
解整合素金屬蛋白酶15(ADAM15)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 15 (ADAM15)48T/96TY12901Homo sapiens (Human)
磷酸肌醇-3-激酶催化亞基δ肽(PIK3Cδ)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Phosphoinositide-3-Kinase Catalytic Delta Polypeptide (PIK3Cd)48T/96TY12902Homo sapiens (Human)
載脂蛋白C3(APOC3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Apolipoprotein C3 (APOC3)48T/96TY12903Mus musculus (Mouse)
胃動蛋白1(GKN1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Gastrokine 1 (GKN1)48T/96TY12904Rattus norvegicus (Rat)
白介素22受體α2(IL2Rα2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Interleukin 22 Receptor Alpha 2 (IL22Ra2)48T/96TY12905Homo sapiens (Human)
?;视图っ福ˋGK)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Acylglycerol Kinase (AGK)48T/96TY12906Homo sapiens (Human)
睪酮(Testosterone)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Testosterone48T/96TY12907General
對氧磷酶1(PON1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Paraoxonase 1 (PON1)48T/96TY12908Mus musculus (Mouse)
鳥氨酸氨基甲酰轉移酶(OCT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Ornithine Carbamoyl Transferase (OCT)48T/96TY12909Bos taurus; Bovine (Cattle)
血管內皮生長因子121(VEGF121)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Vascular Endothelial Growth Factor 121 (VEGF121)48T/96TY12910Oryctolagus cuniculus (Rabbit)
成纖維細胞生長因子11(FGF11)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 11 (FGF11)48T/96TY12911Mus musculus (Mouse)
成纖維細胞生長因子12(FGF12)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 12 (FGF12)48T/96TY12912Mus musculus (Mouse)
WNT1誘導信號通道蛋白1(WISP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for WNT1 Inducible Signaling Pathway Protein 1 (WISP1)48T/96TY12913Homo sapiens (Human)
谷氧還蛋白3(GLRX3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutaredoxin 3 (GLRX3)48T/96TY12914Homo sapiens (Human)
胃蛋白酶原C(PGC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Pepsinogen C (PGC)48T/96TY12915Rattus norvegicus (Rat)
芳香烴受體(AhR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Aryl Hydrocarbon Receptor (AhR)48T/96TY12916Rattus norvegicus (Rat)
N-甲基-D-天氡氨酸離子能谷氨酸受體2A(GRIN2A)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2A (GRIN2A)48T/96TY12917Homo sapiens (Human)
趨化因子C-基元配體2(XCL2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Chemokine C-Motif Ligand 2 (XCL2)48T/96TY12918Homo sapiens (Human)
羧肽酶A3(CPA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase A3, Mast Cell (CPA3)48T/96TY12919Mus musculus (Mouse)
肌苷三磷酸酶(ITPA)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Inosine Triphosphatase (ITPA)48T/96TY12920Mus musculus (Mouse)
Persephin蛋白(PSPN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Persephin (PSPN)48T/96TY12921Mus musculus (Mouse)
平足蛋白(PDPN)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Podoplanin (PDPN)48T/96TY12922Rattus norvegicus (Rat)
半胱氨酸豐富血管生成誘導因子61(CYR61)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61)48T/96TY12923Homo sapiens (Human)
羧肽酶A3(CPA3)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Carboxypeptidase A3, Mast Cell (CPA3)48T/96TY12924Rattus norvegicus (Rat)
卵泡刺激素受體(FSHR)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Follicle Stimulating Hormone Receptor (FSHR)48T/96TY12925Homo sapiens (Human)
骨骼肌慢肌肌鈣蛋白I(TNNI1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I Type 1, Slow Skeletal (TNNI1)48T/96TY12926Rattus norvegicus (Rat)
骨骼肌慢肌肌鈣蛋白I(TNNI1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I Type 1, Slow Skeletal (TNNI1)48T/96TY12927Homo sapiens (Human)
血小板衍生生長因子C(PDGFC)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Plaet Derived Growth Factor C (PDGFC)48T/96TY12928Homo sapiens (Human)
激肽釋放酶9(KLK9)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Kallikrein 9 (KLK9)48T/96TY12929Rattus norvegicus (Rat)
賴氨酰氧化酶(LOX)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Lysyl Oxidase (LOX)48T/96TY12930Mus musculus (Mouse)
皮膚橋蛋白(DPT)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Dermatopontin (DPT)48T/96TY12931Homo sapiens (Human)
骨骼肌快肌肌鈣蛋白I(TNNI2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I Type 2, Fast Skeletal (TNNI2)48T/96TY12932Mus musculus (Mouse)
骨骼肌快肌肌鈣蛋白I(TNNI2)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Troponin I Type 2, Fast Skeletal (TNNI2)48T/96TY12933Rattus norvegicus (Rat)
鋅結合α2-糖蛋白1(αZGP1)檢測試劑盒(酶聯免疫吸附試驗法)ELISA Kit for Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1)48T/96TY12934Homo sapiens (Human)
各種類型ELISA測定時,一般需經過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結果。